Mascherano, 40, is a former teammate of Miami star Lionel Messi. The two were teammates at FC Barcelona from 2010-18.
Miami had won the regular season campaign with a record points total but after losing twice in the best-of-three series, the MLS Cup favourites and their eight-times Ballon d'Or winner are out.
Portuguese winger Xande Silva blasted home a sensational winner for Atlanta in the fourth minute of stoppage time in front of 68,455 home fans at the Mercedes-Benz Stadium to level the best-of-three postseason series 1-1 and set up a winner-take-all game three in Miami next Saturday.
Miami started with all their big-name stars, including Lionel Messi, on the bench but the Argentine was introduced in the 61st minute to the delight of many in the Toronto crowd.
Messi had been subdued until the 45th minute when he put Inter ahead -- chesting down a long ball into the box and getting a lucky bounce off Crew defender Malte Amundsen before poking home.
Messi tied Saturday's match in the 67th minute when he lasered a shot from beyond the box that evaded diving Charlotte goalkeeper Kristijan Kahlina to make it 1-1.
Inter Miami's charge towards Major League Soccer's single-season points record suffered a setback on Saturday after a last-gasp injury-time equalizer from James Sands snatched a 1-1 draw for New York City FC against the Eastern Conference leaders.
The Argentinian superstar Messi came close to a game-clinching goal in the 83rd, but his angled effort was blocked by Atlanta goalkeeper Brad Guzan and Suarez's attempt to tap in the rebound was also denied.
The eight-times Ballon d'Or winner hadn't played since injuring his right ankle during the Copa America final on July 14, when he helped Argentina to victory over Colombia.
Fans of Lionel Messi's Inter Miami are up in arms after the Major League Soccer club doubled season ticket prices for next season, but the chance to watch the World Cup winner will still tempt many into forking out the cash.
Without Messi and his former Barcelona team-mate Jordi Alba, Miami struggled to compete with an enterprising Houston and could have little to complain about the outcome.
Messi's former Barcelona teammate, Spaniard Jordi Alba, is almost certain to be ruled out of the game for Miami but Martino said there remains a chance the Argentine World Cup winner could take some part
Inter Miami, without Lionel Messi, Sergio Busquets and Jordi Alba, battled to a 1-1 draw at Orlando City in Major League Soccer on Sunday.
Inter Miami coach Gerardo "Tata" Martino confirmed Friday that star forward Lionel Messi will miss Sunday's match at Orlando City as he deals with a scar tissue issue from an injury with the Argentina national team.
Messi returned to the starting line-up after skipping Saturday's 5-2 defeat at Atlanta due to muscle fatigue but he had to leave the field in the 38th minute, looking uncomfortable
It isn't yet known if either Messi or Alba will be available Wednesday, though both trained in full on Tuesday. With two regular-season matches slated before Miami plays in the U.S. Open Cup final on Sept. 27, Messi and/or Alba could be given further time to recuperate
Messi and his former Barcelona team-mate Jordi Alba both sat out the game with Martino saying it would have been "very reckless" for the Argentine to play in Atlanta
Inter Miami rested Lionel Messi and suffered their first defeat since the Argentine joined the club, with a 5-2 hammering at Atlanta United damaging their playoff hopes on Saturday.
Messi was one of eight Inter Miami players away on international duty, but Gerardo "Tata" Martino's team showed their strength in depth as they extended their unbeaten run in all competitions to 12 games.
