Neymar was out for more than a year until last month following right knee surgery.
The Brazilian superstar lasted just 26 minutes as a second-half substitute before limping off with an apparent right hamstring strain in Monday's 3-0 AFC Champions League Elite win over Esteghlal.
The evening ended on a sour note for Al-Hilal when Neymar, who returned two weeks ago from a year out due to a ruptured cruciate ligament, was replaced 29 minutes after his introduction from the bench due to injury.
The former Barcelona and Paris Saint-Germain attacker had not played since rupturing his meniscus and anterior cruciate ligament in his left knee while on international duty in October last year.
"Happy to be back in the group," Neymar posted on Instagram, with pictures of him running and kicking the ball, watched by Al Hilal's Portuguese coach Jorge Jesus.
Brazil coach Dorival Junior called for Neymar to be given as much time as he needs to make a complete recovery from a knee injury that has sidelined him for almost a year.
Neymar is set to miss the start of the next Saudi Pro League season as he recovers from a serious knee injury, Al Hilal's coach said Tuesday.
The country of Pele, Garrincha and Ronaldinho, which once wowed the world with its 'samba' style, has not won the World Cup since 2002. Nor has it produced a Ballon d'Or winner since Kaka in 2007.
Jorge Jesus' side, top of the Saudi league and still alive in the King's Cup and Asian Champions league, are bidding for a quadruple of trophies this season.
Brazil will look to secure top spot in Copa America Group B by winning against Columbia, who slipped to a 2-1 defeat against Peru. Securing top spot will help in reducing the chances of coming up against Lionel Messi's Argentina before the final.
Barcelona captain Lionel Messi says Neymar remains determined to return to the club and the Argentine wants him to turn them into an all-conquering force again, adding that he does not believe they are good enough at the moment to win the Champions League.
The two most expensive players in the world were unable to outshine the prolific Erling Braut Haaland as the Norwegian sensation continues his remarkable rise by scoring both goals in Borussia Dortmund's 2-1 defeat of Paris Saint-Germain in their Champions League first-leg encounter
Brazilian Neymar will not play in Sunday's season opener for Paris St Germain, the Ligue 1 club said, as speculation continues to swirl over whether or not the forward will remain at the French champions.
Paris Saint Germain coach Thomas Tuchel revealed that he had known before the Copa America tournament that Neymar had wanted to leave the French club.
French champions Paris St Germain have said they will take action against Neymar after the Brazilian forward failed to report for training on Monday.
Paris St Germain revived their Champions League campaign with a 2-1 win over Liverpool in Group C on Wednesday, leaving the English club's hopes of reaching the last 16 hanging in the balance.
Brazil forward Neymar may have committed his future to French club Paris Saint-Germain but the Brazilian is likely to switch to Spain ‘sooner rather than later’ according to former Brazil player Rivaldo.
The biggest change for Brazil at this World Cup is not on the field, or on the training ground, or even in the dressing-room. It's on the team sheet.
Criticised for being selfish, profligate and over-dramatic, Neymar struggles to such an extent at this World Cup.