The reigning world champions will also be without Angel Di Maria after the veteran winger's international retirement.
AS Roma forward Paulo Dybala said it was a big blow to be left out of Argentina's Copa America squad and that he had been confident of selection for the tournament in the United States.
Paulo Dybala will miss Argentina's upcoming friendlies with El Salvador and Costa Rica after his club Roma announced on Saturday that the forward had picked up a thigh injury.
"The talent of the champion came out. Players like Paulo are decisive and for me they should never be questioned," said Roma coach Daniele De Rossi.
Paulo Dybala scored twice as Roma eased to a 4-0 thumping of Cagliari at the Stadio Olimpico on Monday to notch a third straight Serie A win under Daniele De Rossi.
Injured forward Paulo Dybala made the cut as Argentina coach Lionel Scaloni named on Friday his 26-man squad for the Nov. 20-Dec. 18 World Cup in Qatar, where Lionel Messi will lead the side.
Paulo Dybala shot Roma top of Serie A on Tuesday with a brace which sank Monza 3-0 while Inter Milan are one point behind Jose Mourinho's side after their 3-1 home win over Cremonese.
Paulo Dybala was left stunned when he was unveiled in front of around 10000 Roma fans at the Square Colosseum on Tuesday.
AS Roma forward Paulo Dybala said on Tuesday his contract with former club Juventus had not been renewed because he was not part of the Serie A club's future plans while thousands of AS Roma fans welcomed him in the Italian capital.
Argentina international forward Paulo Dybala has agreed a deal in principle to sign for Serie A side AS Roma on a free transfer following his departure from Juventus, a source close to the club told Reuters on Monday
Paulo Dybala said that he believes he is ready to feature for Argentina and play beside ‘the best player in the world’Lionel Messi. Dybala was left on the bench as Argentina were held 1-1against Iceland in their opening match.
Paulo Dybala strikes twice to send Juventus to the top of Serie A with a 2-0 victory over Udinese as title rivals Napoli are held to a goalless draw at Inter Milan.
Paulo Dybala should avoid worrying about comparisons with Lionel Messi and Cristiano Ronaldo if he wants to become the best player in the world, says Juventus coach Massimiliano Allegri.
Barcelona playmaker Lionel Messi gives an interview with TyC Sports where he breaks silence on the claims that he runs the Argentina squad selection process.
Paulo Dybala, who is one of the most exciting young players in the world right now may have snubbed Barcelona due to the love of the number 10 shirt.
Paulo Dybala grabs a hat-trick to give Juventus a dramatic 4-2 win at Genoa as the Serie A titleholders recover from going two goals down in the first seven minutes.
Diminutive, dynamic and armed with a deadly left foot, Paulo Dybala could be a prototype of a Lionel Messi clone.
Juventus are optimistic that forward Paulo Dybala will recover from injury in time to face Barcelona in the Champions League on Wednesday after he was forced out of Saturday's game with Pescara.