Dinesh Karthik announced his retirement from all cricket on social media, bringing an end to a professional career that began in the early 2000s.
"As you can see jersey still fits so if they need an extra man, I can be the impact player. It is good to see the fans. RCB forever, I will forever be an RCB fan," Gayle, the RCB Hall of Fame, said in a video posted by RCB on X.
"What a night! Such a great atmosphere," player of the match Du Plessis, who did a lap of honour with his team for the home fans, said.
"We want to play that style of cricket - RCB talks about playing bold," Du Plessis said. "Just proud that we can actually do that now, putting our performance together."
Kohli's strike rate and his effectiveness against spin bowling had become a hot topic of debate in recent weeks.
Chasing a modest 148 for victory, Du Plessis, who hit 64, and Kohli, who made 42, laid the foundations with a stand of 92 in 35 balls
Hyderabad could only score 171-8 as RCB registered only their second win in nine games and are still languishing at the bottom of the 10-team table.
The match produced the highest aggregate for a T20 game with a tally of 549 runs.
The ace Indian pacer's masterful 5-21 won him the player-of-the-match and restricted the star-studded RCB line up to 196-8 after Hardik Pandya won the toss and decided to field first at the home ground in Mumbai.
India's leading batsman Virat Kohli has been rested from the limited-overs series against Zimbabwe, scheduled from June 11 to 20, along with Murali Vijay and Ashish Nehra.
When Kohli and ABD are on song, there's not much the opposition can do. And if Chris Gayle joins the party, the captain at the other end runs out of options pretty quickly.
Self-styled "Universe Boss" Chris Gayle is having to confront his waning powers after a collapse in form, watching from the bench as Virat Kohli takes on his mantle as cricket's superstar batsman.
A flurry of boundaries from Kieron Pollard and Jos Buttler guide Mumbai Indians to a six-wicket victory over Royal Challengers Bangalore, their sixth victory of the competition at the Chinnaswami Stadium.