The 39-year-old winger -- Sevilla and the Spanish national team's most-decorated player -- will officially retire when his contract expires on December 31 due to a painful and chronic hip injury which has plagued him in recent seasons.
Kylian Mbappe scored a screamer and set up another goal as Real Madrid thrashed Sevilla 4-2 on Saturday to move second in La Liga above stuttering rivals Barcelona.
"Sergio Ramos has informed Sevilla FC that he will not stay next season," the club said in a statement.
The player's contract was set to expire at the end of the campaign.
Antoine Griezmann struck a hat-trick in a 3-0 win over 10-man Celta Vigo to help Atletico Madrid climb provisionally second, in hot pursuit of their city rivals.
Sergio Ramos has not played against Real Madrid since 2005 but on Saturday, his Sevilla team welcome the Spanish league leaders to the Sanchez-Pizjuan for a reunion.
Al-Hilal have signed Morocco goalkeeper Yassine Bounou from Spanish side Sevilla on a three-year contract, the Saudi Pro League club said on Thursday.
His message was one of pride, which is what he has restored at Roma, a team which has one of Italy's largest, most passionate fan bases but has won little of note since being founded in 1927.
Sevilla beat AS Roma 4-1 on penalties on Wednesday to win the Europa League for a record-extending seventh time, handing Jose Mourinho his first defeat in six European finals.
Atletico Madrid says they had signed Spanish international Victor Machin Perez, know as 'Vitolo', from Sevilla but immediately loaned him to Las Palmas to comply with a FIFA recruitment ban.
Leicester City say their signing of Vicente Iborra from Sevilla would be a four-year deal and they have agreed terms, with the fee reported to be in the region of 12 million euros (10.5 million pounds).
Eduardo Berizzo will take charge of Sevilla next season after the Spanish club announce the agreement of a two-year deal with the Argentine.
Jorge Sampaoli reacted angrily on Friday to Sevilla's decision to threaten the Argentine Football Association (AFA) with legal action over AFA's interest in hiring the Sevilla coach to rescue their floundering World Cup qualifying campaign.
Jorge Sampaoli has a final shot to further his case to land his dream job at the end of the season when his slumping Sevilla side travel to Barcelona on Wednesday.
Sevilla midfielder Samir Nasri has accused Jamie Vardy of cheating after the Frenchman was sent off for squaring up to the Leicester forward and bumping foreheads in their Champions League clash on Tuesday.
Sevilla goalkeeper Sergio Rico blundered and then made two brilliant late stops as they were held to a 1-1 draw at Alaves on Monday that dented their La Liga title bid.
Sevilla captain Vicente Iborra came off the bench to lead his side to a second-half comeback at fierce local rivals Real Betis on Saturday, assisting and scoring in a 2-1 win to provisionally take Jorge Sampaoli's side joint-top of La Liga.
Sevilla left-back Sergio Escudero was rewarded for his fine form this season with a new four-year contract, the Spanish club confirmed on Thursday.
Leicester City need to wipe last year's incredible title win from their memories and focus on turning their poor season around, starting with their Champions League last-16 first leg at Sevilla, striker Jamie Vardy said on Tuesday.
