"Sergio Ramos has informed Sevilla FC that he will not stay next season," the club said in a statement.
The player's contract was set to expire at the end of the campaign.
Antoine Griezmann struck a hat-trick in a 3-0 win over 10-man Celta Vigo to help Atletico Madrid climb provisionally second, in hot pursuit of their city rivals.
Sergio Ramos has not played against Real Madrid since 2005 but on Saturday, his Sevilla team welcome the Spanish league leaders to the Sanchez-Pizjuan for a reunion.
Al-Hilal have signed Morocco goalkeeper Yassine Bounou from Spanish side Sevilla on a three-year contract, the Saudi Pro League club said on Thursday.
His message was one of pride, which is what he has restored at Roma, a team which has one of Italy's largest, most passionate fan bases but has won little of note since being founded in 1927.
Sevilla beat AS Roma 4-1 on penalties on Wednesday to win the Europa League for a record-extending seventh time, handing Jose Mourinho his first defeat in six European finals.
Marcus Rashford will miss Manchester United's Europa League quarter-final first-leg clash with Sevilla after suffering a muscle injury that is likely to keep him out for "a few games", the club announced on Wednesday.
European soccer's governing body UEFA has opened an investigation after Sevilla goalkeeper Marko Dmitrovic was attacked by a fan on the pitch in Thursday's Europa League match at PSV Eindhoven, The Athletic sports website reported.
Eduardo Berizzo will take charge of Sevilla next season after the Spanish club announce the agreement of a two-year deal with the Argentine.
Jorge Sampaoli reacted angrily on Friday to Sevilla's decision to threaten the Argentine Football Association (AFA) with legal action over AFA's interest in hiring the Sevilla coach to rescue their floundering World Cup qualifying campaign.
Jorge Sampaoli has a final shot to further his case to land his dream job at the end of the season when his slumping Sevilla side travel to Barcelona on Wednesday.
Sevilla midfielder Samir Nasri has accused Jamie Vardy of cheating after the Frenchman was sent off for squaring up to the Leicester forward and bumping foreheads in their Champions League clash on Tuesday.
Sevilla goalkeeper Sergio Rico blundered and then made two brilliant late stops as they were held to a 1-1 draw at Alaves on Monday that dented their La Liga title bid.
Sevilla captain Vicente Iborra came off the bench to lead his side to a second-half comeback at fierce local rivals Real Betis on Saturday, assisting and scoring in a 2-1 win to provisionally take Jorge Sampaoli's side joint-top of La Liga.
Sevilla left-back Sergio Escudero was rewarded for his fine form this season with a new four-year contract, the Spanish club confirmed on Thursday.
Leicester City need to wipe last year's incredible title win from their memories and focus on turning their poor season around, starting with their Champions League last-16 first leg at Sevilla, striker Jamie Vardy said on Tuesday.
Samir Nasri missed a second-half penalty as Sevilla squandered the chance to close to within a point of La Liga leaders Real Madrid with a 0-0 draw home to Villarreal on Sunday.
Sevilla made Argentine midfielder Walter Montoya their third signing of the January transfer window on Saturday on a four-and-a-half year deal from Rosario Central.
