Lewandowski suffered a back injury in Sunday's 1-0 defeat at Real Sociedad during which he had a goal controversially disallowed.
Lewandowski's early disallowed strike led to furious debate over whether the Polish forward had been offside or not.
Barcelona coach Hansi Flick said he thought playmaker Dani Olmo would be ready despite feeling unwell overnight, while defender Pau Cubarsi can play despite suffering a cut on his face during the team's Champions League win over Red Star Belgrade on Wednesday.
It was another commanding victory for LaLiga leaders Barcelona who are in an imperious vein of form, scoring 24 goals in six successive wins in all competitions, including thrashings of Bayern Munich and Real Madrid.
Dani Olmo struck twice for Barcelona on his first start after an injury absence as they beat local rivals Espanyol 3-1 on Sunday to move nine points clear at the top of La Liga.
"Honestly I don't see it as extraordinary, even if some people do... when I was little I also looked on from the outside, watching from my house seeing Messi lift Ballons d'Or and thinking it was incredible. Once you are on the inside, you take it normally, naturally, although if I stop to think about it, it's something really big."
The Catalan giants triumphed at the Santiago Bernabeu to snap Madrid's run of 42 matches unbeaten and continue their own revival under the German coach who arrived this summer.
Defending champions Real Madrid are set to host archrivals Barcelona in a LaLiga game at the Santiago Bernabeu tonight.
Kylian Mbappe's start at Real Madrid has been satisfactory, his performances neither spectacular nor underwhelming, but Saturday's La Liga Clasico offers the French superstar a springboard to kick on from.
As part of their sponsorship agreement with music streaming platform Spotify, Barcelona have worn logos of rock band the Rolling Stones, and artists Drake and Rosalia in previous Clasicos.
Andreas Christensen came off the bench to head in the winning goal as Barcelona won 3-2 away to Paris Saint-Germain in a remarkable first leg of their Champions League quarterfinal tie on Wednesday.
The French government said there was no "concrete threat" to Paris Saint-Germain's Champions League match against Barcelona on Wednesday in the French capital after Islamic State threatened to attack all four quarterfinals.
Dembele was in a Barcelona shirt the last time the clubs met, in the last 16 in 2021, when Mbappe scored a lethal hat-trick in the first leg in Catalonia
“I am ready and, as always, I am not going to hide. Seeing the dynamic we have, I'm sure we're going to give it our all, then the result... It's in God's hands,” Mbappe told Telefoot
A second-half header by winger Raphinha earned second-placed Barcelona a 1-0 win over Las Palmas in LaLiga on Saturday after the visitors were reduced to 10 men in the first half.
Barcelona coach Xavi Hernandez said Friday teenage stars Lamine Yamal and Pau Cubarsi could go on to 'define an era' of world football.
Convicted rapist and former Brazil international footballer Dani Alves will remain in jail until at least Monday after failing to raise the one-million-euro bail set by a Barcelona court to ensure his release pending appeal.
Former Brazil international footballer Dani Alves is to be released from jail pending an appeal against his rape conviction in exchange for posting bail of one million euros ($1.08 million), a Barcelona court said Wednesday.
Lewandowski set up goals for Joao Felix and Fermin Lopez either side of striking himself to pull Barcelona second, eight points behind leaders Real Madrid.