Neymar was out for more than a year until last month following right knee surgery.
The Brazilian superstar lasted just 26 minutes as a second-half substitute before limping off with an apparent right hamstring strain in Monday's 3-0 AFC Champions League Elite win over Esteghlal.
The evening ended on a sour note for Al-Hilal when Neymar, who returned two weeks ago from a year out due to a ruptured cruciate ligament, was replaced 29 minutes after his introduction from the bench due to injury.
The former Barcelona and Paris Saint-Germain attacker had not played since rupturing his meniscus and anterior cruciate ligament in his left knee while on international duty in October last year.
"Happy to be back in the group," Neymar posted on Instagram, with pictures of him running and kicking the ball, watched by Al Hilal's Portuguese coach Jorge Jesus.
Brazil coach Dorival Junior called for Neymar to be given as much time as he needs to make a complete recovery from a knee injury that has sidelined him for almost a year.
Neymar is set to miss the start of the next Saudi Pro League season as he recovers from a serious knee injury, Al Hilal's coach said Tuesday.
The country of Pele, Garrincha and Ronaldinho, which once wowed the world with its 'samba' style, has not won the World Cup since 2002. Nor has it produced a Ballon d'Or winner since Kaka in 2007.
Jorge Jesus' side, top of the Saudi league and still alive in the King's Cup and Asian Champions league, are bidding for a quadruple of trophies this season.
PSG striker Edison Cavani denies there is a rift between him and PSG’s Brazilian forward Neymar despite signs that say otherwise after the two disputed over free-kicks and penalty duties during PSG's win over Lyon.
Brazilian newspaper Estadao report says that Neymar has advised Coutinho against a Barca move.
Neymar will be denied an immediate return to Barcelona when the Champions League draw is made in Monaco on Thursday.
Barcelona players participated in a team lunch, which was also attended by coach Ernesto Valverde to discuss their immediate future, Sport newspaper reports.
Barcelona star Lionel Messi reunites with former teammate Neymar and posts a picture of the MSN trio on instagram with a message for defender Gerard Pique.
Paris Saint-Germain's world record signing Neymar launches a scathing attack on his former club's directors, saying "Barca deserve much better".
The world of sport united to show solidarity with the victims of the Barcelona attack and condemned the incident.
Lionel Messi reportedly promised that he would make Neymar a Ballon d'Or winner if he stayed back at the Camp Nou.
Gerard Pique knew Neymar had decided to leave Barcelona long before the Brazilian completed his world record 222 million euro ($261 million) move to Paris Saint-Germain, the Barcelona defender admits at a press conference.
Neymar should be free to make his Paris Saint-Germain debut at Guingamp on Sunday after Barcelona confirm the 222 million-euro ($261 million) cheque used to pay his buyout clause had been cleared.