Neymar was out for more than a year until last month following right knee surgery.
The Brazilian superstar lasted just 26 minutes as a second-half substitute before limping off with an apparent right hamstring strain in Monday's 3-0 AFC Champions League Elite win over Esteghlal.
The evening ended on a sour note for Al-Hilal when Neymar, who returned two weeks ago from a year out due to a ruptured cruciate ligament, was replaced 29 minutes after his introduction from the bench due to injury.
The former Barcelona and Paris Saint-Germain attacker had not played since rupturing his meniscus and anterior cruciate ligament in his left knee while on international duty in October last year.
"Happy to be back in the group," Neymar posted on Instagram, with pictures of him running and kicking the ball, watched by Al Hilal's Portuguese coach Jorge Jesus.
Brazil coach Dorival Junior called for Neymar to be given as much time as he needs to make a complete recovery from a knee injury that has sidelined him for almost a year.
Neymar is set to miss the start of the next Saudi Pro League season as he recovers from a serious knee injury, Al Hilal's coach said Tuesday.
The country of Pele, Garrincha and Ronaldinho, which once wowed the world with its 'samba' style, has not won the World Cup since 2002. Nor has it produced a Ballon d'Or winner since Kaka in 2007.
Jorge Jesus' side, top of the Saudi league and still alive in the King's Cup and Asian Champions league, are bidding for a quadruple of trophies this season.
Brazil have omitted West Ham United midfielder Lucas Paqueta from their squad for next month's World Cup qualifiers amid media reports that the English FA and FIFA have opened an investigation into potential betting rule breaches.
A rock star welcome will await him at the King Fahd International Stadium in Riyadh where he will be unveiled before thousands of fans on Saturday.
The 31-year-old Neymar signed a two-year contract with the Riyadh-based club on Tuesday after spending six years with Paris St Germain, in a deal reportedly worth about 90 million euros ($98 million)
Cristiano Ronaldo's arrival in January to play for Riyadh-based Al-Nassr is what first drew global attention to ongoing efforts to boost the Saudi Pro League.
Saudi Professional League (SPL) club Al-Hilal announced that Neymar had signed a contract until 2025, with the Brazilian saying, "I am here in Saudi Arabia, I am Hilali" in a video on the team's social media accounts
The BBC reported the transfer fee to be about 90 million euros ($98.56 million) plus add-ons.
A source close to PSG told AFP that 31-year-old Neymar "no longer figures in the club or manager's plans" with the Brazilian immediately linked to Al-Hilal in the Gulf kingdom
The Brazil international joined the French champions for 220 million euros ($241m) from Barcelona in 2017
Also missing against Lorient is Brazilian Neymar who is sidelined with a "viral syndrome".
Neymar has told Paris Saint-Germain that he wants to leave the French club this summer as he aims to make a sensational return to Barcelona, according to reports.