"We have been extraordinarily extraordinary in the past, but not any more," Guardiola told a press conference on Wednesday.
Real Madrid have lost just one of their six Champions League home games against City (W3 D2), going down 2-1 in the round of 16 in 2019-20.
His team trail after a late collapse saw Real beat them 3-2 at the Etihad in the first leg last week.
European champions Madrid hold a 3-2 lead on City, who have struggled this season and are fourth in the Premier League, ahead of the play-off round second leg on Wednesday at the Santiago Bernabeu.
Ancelotti was delighted to finally lead Real to their first success at the Etihad Stadium, a victory he thought was deserved due to the chances created, with Real having 20 shots compared to 11 from City, despite falling behind twice.
Real and City are set to clash for the fourth consecutive season in Europe's elite club competition, with the first leg in Manchester on Tuesday.
"He will not be included in the squad for the match in Manchester," said Madrid in a statement on Sunday, with Spanish media reporting he will miss three weeks.
It was sweet revenge for Real Madrid, who were thrashed 4-0 at the Etihad Stadium in their exit from the Champions League at the semi-final stage last year.
Real midfielder Federico Valverde was named UEFA's man-of-the-match, but Lunin had made a strong case to be handed the award after making two saves in their 4-3 shootout win after an inspired performance as they drew their quarter-final second leg 1-1.
Last season record 14-time winners Madrid tied 1-1 with Pep Guardiola's City at home in the semi-final first leg but were hammered 4-0 at the Etihad Stadium.
"We competed, we fought, I think we deserved to win. It was a good match and we head to the second leg with a good feeling. The result doesn't reward us for what we did on the pitch, but this tie will be close until the last minute," Ancelotti told Movistar Plus.
The reigning 14-time record champions host Pep Guardiola's hungry visitors in the first leg who are spearheaded by Norwegian hitman Haaland, who boasts 51 goals in 45 games across all competitions
