Neymar was out for more than a year until last month following right knee surgery.
The Brazilian superstar lasted just 26 minutes as a second-half substitute before limping off with an apparent right hamstring strain in Monday's 3-0 AFC Champions League Elite win over Esteghlal.
The evening ended on a sour note for Al-Hilal when Neymar, who returned two weeks ago from a year out due to a ruptured cruciate ligament, was replaced 29 minutes after his introduction from the bench due to injury.
The former Barcelona and Paris Saint-Germain attacker had not played since rupturing his meniscus and anterior cruciate ligament in his left knee while on international duty in October last year.
"Happy to be back in the group," Neymar posted on Instagram, with pictures of him running and kicking the ball, watched by Al Hilal's Portuguese coach Jorge Jesus.
Brazil coach Dorival Junior called for Neymar to be given as much time as he needs to make a complete recovery from a knee injury that has sidelined him for almost a year.
Neymar is set to miss the start of the next Saudi Pro League season as he recovers from a serious knee injury, Al Hilal's coach said Tuesday.
The country of Pele, Garrincha and Ronaldinho, which once wowed the world with its 'samba' style, has not won the World Cup since 2002. Nor has it produced a Ballon d'Or winner since Kaka in 2007.
Jorge Jesus' side, top of the Saudi league and still alive in the King's Cup and Asian Champions league, are bidding for a quadruple of trophies this season.
A leaner and fitter Lionel Messi, similarly rejuvenated Neymar and Kylian Mbappe staying after signing a new contract makes Paris St Germain runaway favourites to retain their Ligue 1 title when the French league season kicks off on Friday.
A Spanish prosecutor requested a two-year jail term and a 10 million euro ($10.20 million) fine for Brazilian striker Neymar in an embezzlement case against him and his agents over his 2013 transfer to Barcelona soccer club, a court document released on Friday showed.
Neymar is due to face trial in October over alleged irregularities in his transfer to Barcelona, judicial sources said on Wednesday.
Neymar may have produced the worst dive of his career during Paris Saint-Germain's friendly with Gamba Osaka on Monday.
Lionel Messi and Neymar combined to devastating effect as Paris Saint-Germain thrashed Gamba Osaka 6-2 on Monday to finish their pre-season Japan tour with three wins out of three.
Brazil manager Tite is excited by the rise of the young players who surged to stardom in European soccer last season and will be available for the national team in November when his side bid to win a record-extending sixth World Cup at the finals in Qatar.
Neymar said Saturday that he wants to stay with Paris Saint-Germain and does not "need to prove" himself to anyone after five years with the French club.
Kylian Mbappe scored as Paris Saint-Germain made it two wins out of two on their Japanese tour with a 3-0 victory over J-League side Urawa Reds on Saturday.
Lionel Messi, Neymar and Kylian Mbappe drew gasps from a crowd of thousands in Tokyo on Monday as Paris Saint-Germain held the first training session of their Japanese tour
Paris Saint-Germain's star-studded squad landed in Japan Sunday for a three-game tour against local teams, with new manager Christophe Galtier hoping to make a strong start with the French giants.