Neymar was out for more than a year until last month following right knee surgery.
The Brazilian superstar lasted just 26 minutes as a second-half substitute before limping off with an apparent right hamstring strain in Monday's 3-0 AFC Champions League Elite win over Esteghlal.
The evening ended on a sour note for Al-Hilal when Neymar, who returned two weeks ago from a year out due to a ruptured cruciate ligament, was replaced 29 minutes after his introduction from the bench due to injury.
The former Barcelona and Paris Saint-Germain attacker had not played since rupturing his meniscus and anterior cruciate ligament in his left knee while on international duty in October last year.
"Happy to be back in the group," Neymar posted on Instagram, with pictures of him running and kicking the ball, watched by Al Hilal's Portuguese coach Jorge Jesus.
Brazil coach Dorival Junior called for Neymar to be given as much time as he needs to make a complete recovery from a knee injury that has sidelined him for almost a year.
Neymar is set to miss the start of the next Saudi Pro League season as he recovers from a serious knee injury, Al Hilal's coach said Tuesday.
The country of Pele, Garrincha and Ronaldinho, which once wowed the world with its 'samba' style, has not won the World Cup since 2002. Nor has it produced a Ballon d'Or winner since Kaka in 2007.
Jorge Jesus' side, top of the Saudi league and still alive in the King's Cup and Asian Champions league, are bidding for a quadruple of trophies this season.
Several hundred PSG supporters gathered outside club headquarters on Wednesday evening where they set off smoke flares and sang hostile chants about underperforming stars Messi, Neymar and Italian midfielder Marco Verratti
A brilliant Lionel Messi goal helped Paris Saint-Germain to a 3-1 home win over Lens on Saturday to surely end any doubt about the outcome of this season's Ligue 1 title race, after an early sending-off left the visitors with a mountain to climb.
New France skipper Kylian Mbappe said he would not change after being given the armband by coach Didier Deschamps for the Euro 2024 qualifiers as he promised to be "a different captain" to long-term predecessor Hugo Lloris.
Paris St Germain suffered yet another setback as they slumped to their first Ligue 1 home defeat of the season when they were beaten 2-0 by Stade Rennais on Sunday.
Doctors at the Aspetar hospital would not give a date when the 31-year-old Paris Saint-Germain striker would return to action but they are confident that Friday's surgery was a success
Paris Saint-German forward Neymar arrived in Doha on Friday for an operation on the ankle injury that has ended his season, Qatar's official news agency said.
Bayern Munich host Paris Saint-Germain on Wednesday with the stage set for star turns from their 2022-23 additions Sadio Mane and Matthijs de Ligt.
Neymar tweeted he would not be discouraged by his latest injury
The Brazilian, who suffered the injury on February 19 against Lille in Ligue 1, "has had several episodes of instability in the right ankle in recent years," said the club, adding that its medical staff "recommended a ligament repair operation" be undertaken in Doha
"New examinations taken today confirm a sprained ankle for Neymar with ligament damage," PSG said in a statement